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An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Overview of attention for article published in Malaria Journal, February 2012
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Title
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
Published in
Malaria Journal, February 2012
DOI 10.1186/1475-2875-11-42
Pubmed ID
Authors

Sandra Hasenkamp, Eleanor H Wong, Paul Horrocks

Abstract

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 3%
Unknown 39 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 35%
Researcher 7 18%
Student > Bachelor 4 10%
Student > Master 4 10%
Student > Postgraduate 2 5%
Other 5 13%
Unknown 4 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 45%
Biochemistry, Genetics and Molecular Biology 8 20%
Chemistry 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Medicine and Dentistry 2 5%
Other 4 10%
Unknown 3 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2012.
All research outputs
#15,243,549
of 22,665,794 outputs
Outputs from Malaria Journal
#4,454
of 5,540 outputs
Outputs of similar age
#164,090
of 248,335 outputs
Outputs of similar age from Malaria Journal
#50
of 65 outputs
Altmetric has tracked 22,665,794 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,540 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 248,335 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 65 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.