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MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues

Overview of attention for article published in Genome Biology, February 2003
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Title
MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues
Published in
Genome Biology, February 2003
DOI 10.1186/gb-2003-4-3-r17
Pubmed ID
Authors

Seth Blackshaw, Winston P Kuo, Peter J Park, Motokazu Tsujikawa, Jenny M Gunnersen, Hamish S Scott, Wee-Ming Boon, Seong-Seng Tan, Constance L Cepko

Abstract

Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate. We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals. MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 6%
Brazil 1 3%
Unknown 30 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 33%
Student > Ph. D. Student 7 21%
Professor 6 18%
Other 3 9%
Professor > Associate Professor 2 6%
Other 3 9%
Unknown 1 3%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 61%
Medicine and Dentistry 4 12%
Biochemistry, Genetics and Molecular Biology 3 9%
Social Sciences 1 3%
Neuroscience 1 3%
Other 2 6%
Unknown 2 6%