Title |
Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests
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Published in |
BMC Microbiology, May 2012
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DOI | 10.1186/1471-2180-12-83 |
Pubmed ID | |
Authors |
Vicky Jespers, Joris Menten, Hilde Smet, Sabrina Poradosú, Saïd Abdellati, Rita Verhelst, Liselotte Hardy, Anne Buvé, Tania Crucitti |
Abstract |
The vaginal microbiome plays an important role in urogenital health. Quantitative real time Polymerase Chain Reaction (qPCR) assays for the most prevalent vaginal Lactobacillus species and bacterial vaginosis species G. vaginalis and A. vaginae exist, but qPCR information regarding variation over time is still very limited. We set up qPCR assays for a selection of seven species and defined the temporal variation over three menstrual cycles in a healthy Caucasian population with a normal Nugent score. We also explored differences in qPCR data between these healthy women and an 'at risk' clinic population of Caucasian, African and Asian women with and without bacterial vaginosis (BV), as defined by the Nugent score. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 25% |
Chile | 1 | 25% |
Spain | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 50% |
Scientists | 2 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
South Africa | 2 | <1% |
Slovenia | 1 | <1% |
Australia | 1 | <1% |
Estonia | 1 | <1% |
Madagascar | 1 | <1% |
Unknown | 206 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 40 | 19% |
Student > Ph. D. Student | 30 | 14% |
Student > Master | 30 | 14% |
Student > Bachelor | 24 | 11% |
Student > Doctoral Student | 10 | 5% |
Other | 31 | 14% |
Unknown | 50 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 44 | 20% |
Agricultural and Biological Sciences | 44 | 20% |
Biochemistry, Genetics and Molecular Biology | 31 | 14% |
Immunology and Microbiology | 20 | 9% |
Engineering | 5 | 2% |
Other | 16 | 7% |
Unknown | 55 | 26% |