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Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test

Overview of attention for article published in Veterinary Research, June 2012
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

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2 X users
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2 patents

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41 Mendeley
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Title
Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test
Published in
Veterinary Research, June 2012
DOI 10.1186/1297-9716-43-48
Pubmed ID
Authors

Qianqian Hu, Weiye Chen, Kehe Huang, Michael D Baron, Zhigao Bu

Abstract

Peste des petits ruminants virus (PPRV) causes high mortality in goats and sheep and the disease has shown a greatly increased geographic distribution over the last 15 years. It is responsible for serious socioeconomic problems in some of the poorest developing countries. The ability to create recombinant PPRV would provide a useful tool for investigating the biology of the virus and the pathology of disease, as well as for developing new vaccines and diagnostic methods. Here we report the first successful rescue of recombinant PPRV from a full-length cDNA clone of the virus genome. Successful recovery of PPRV was achieved by using a RNA polymerase II promoter to drive transcription of the full-length virus antigenome. We have used this technique to construct a virus expressing a tracer protein (green fluorescent protein, GFP). The recombinant virus replicated as well as the parental virus and could stably express GFP during at least 10 passages. The newly established reverse genetics system for PPRV provides a novel method for constructing a vaccine using PPRV as a vector, and will also prove valuable for fundamental research on the biology of the virus. We found that our recombinant virus allowed more rapid and higher throughput assessment of PPRV neutralization antibody titer via the virus neutralization test (VNT) compared with the traditional method.

X Demographics

X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Algeria 1 2%
Peru 1 2%
Unknown 39 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 20%
Student > Ph. D. Student 7 17%
Student > Master 4 10%
Student > Postgraduate 3 7%
Student > Bachelor 2 5%
Other 4 10%
Unknown 13 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 29%
Veterinary Science and Veterinary Medicine 7 17%
Biochemistry, Genetics and Molecular Biology 3 7%
Nursing and Health Professions 1 2%
Immunology and Microbiology 1 2%
Other 3 7%
Unknown 14 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2014.
All research outputs
#5,151,669
of 25,380,192 outputs
Outputs from Veterinary Research
#224
of 1,323 outputs
Outputs of similar age
#33,469
of 176,831 outputs
Outputs of similar age from Veterinary Research
#1
of 11 outputs
Altmetric has tracked 25,380,192 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,323 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 176,831 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.