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Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress

Overview of attention for article published in Journal of Nanobiotechnology, November 2011
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Title
Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress
Published in
Journal of Nanobiotechnology, November 2011
DOI 10.1186/1477-3155-9-49
Pubmed ID
Authors

Malli Mohan Ganesh Babu, Jayavel Sridhar, Paramasamy Gunasekaran

Abstract

Silver nanoparticles (AgNPs) were synthesized using Bacillus cereus strains. Earlier, we had synthesized monodispersive crystalline silver nanoparticles using B. cereus PGN1 and ATCC14579 strains. These strains have showed high level of resistance to silver nitrate (1 mM) but their global transcriptomic response has not been studied earlier. In this study, we investigated the cellular and metabolic response of B. cereus ATCC14579 treated with 1 mM silver nitrate for 30 & 60 min. Global expression profiling using genomic DNA microarray indicated that 10% (n = 524) of the total genes (n = 5234) represented on the microarray were up-regulated in the cells treated with silver nitrate. The majority of genes encoding for chaperones (GroEL), nutrient transporters, DNA replication, membrane proteins, etc. were up-regulated. A substantial number of the genes encoding chemotaxis and flagellar proteins were observed to be down-regulated. Motility assay of the silver nitrate treated cells revealed reduction in their chemotactic activity compared to the control cells. In addition, 14 distinct transcripts overexpressed from the 'empty' intergenic regions were also identified and proposed as stress-responsive non-coding small RNAs.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Russia 1 2%
Italy 1 2%
Unknown 49 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 27%
Student > Postgraduate 7 13%
Student > Master 7 13%
Researcher 5 10%
Professor 3 6%
Other 9 17%
Unknown 7 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 40%
Biochemistry, Genetics and Molecular Biology 10 19%
Immunology and Microbiology 5 10%
Chemistry 2 4%
Business, Management and Accounting 1 2%
Other 5 10%
Unknown 8 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 August 2012.
All research outputs
#13,867,298
of 22,668,244 outputs
Outputs from Journal of Nanobiotechnology
#465
of 1,386 outputs
Outputs of similar age
#90,376
of 142,891 outputs
Outputs of similar age from Journal of Nanobiotechnology
#4
of 5 outputs
Altmetric has tracked 22,668,244 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,386 research outputs from this source. They receive a mean Attention Score of 3.6. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 142,891 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one.