Title |
Efficacy of bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR for detecting neonatal sepsis: a case control study
|
---|---|
Published in |
BMC Pediatrics, July 2010
|
DOI | 10.1186/1471-2431-10-53 |
Pubmed ID | |
Authors |
Makoto Fujimori, Ken Hisata, Satoru Nagata, Nobuaki Matsunaga, Mitsutaka Komatsu, Hiromichi Shoji, Hiroaki Sato, Yuichiro Yamashiro, Takashi Asahara, Koji Nomoto, Toshiaki Shimizu |
Abstract |
Neonatal sepsis is difficult to diagnose and pathogens cannot be detected from blood cultures in many cases. Development of a rapid and accurate method for detecting pathogens is thus essential. The main purpose of this study was to identify etiological agents in clinically diagnosed neonatal sepsis using bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR (BrRNA-RT-qPCR) and to conduct comparisons with the results of conventional blood culture. Since BrRNA-RT-qPCR targets bacterial ribosomal RNA, detection rates using this approach may exceed those using conventional PCR. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Israel | 1 | 1% |
India | 1 | 1% |
Saudi Arabia | 1 | 1% |
Unknown | 73 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 18% |
Researcher | 9 | 12% |
Student > Master | 8 | 11% |
Student > Bachelor | 7 | 9% |
Other | 7 | 9% |
Other | 16 | 21% |
Unknown | 15 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 25 | 33% |
Agricultural and Biological Sciences | 15 | 20% |
Biochemistry, Genetics and Molecular Biology | 6 | 8% |
Nursing and Health Professions | 3 | 4% |
Immunology and Microbiology | 3 | 4% |
Other | 7 | 9% |
Unknown | 17 | 22% |