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X Demographics
Mendeley readers
Attention Score in Context
| Title |
c-MycERTAM transgene silencing in a genetically modified human neural stem cell line implanted into MCAo rodent brain
|
|---|---|
| Published in |
BMC Neuroscience, July 2009
|
| DOI | 10.1186/1471-2202-10-86 |
| Pubmed ID | |
| Authors |
Lara Stevanato, Randolph L Corteling, Paul Stroemer, Andrew Hope, Julie Heward, Erik A Miljan, John D Sinden |
| Abstract |
The human neural stem cell line CTX0E03 was developed for the cell based treatment of chronic stroke disability. Derived from fetal cortical brain tissue, CTX0E03 is a clonal cell line that contains a single copy of the c-mycERTAM transgene delivered by retroviral infection. Under the conditional regulation by 4-hydroxytamoxifen (4-OHT), c-mycERTAM enabled large-scale stable banking of the CTX0E03 cells. In this study, we investigated the fate of this transgene following growth arrest (EGF, bFGF and 4-OHT withdrawal) in vitro and following intracerebral implantation into a mid-cerebral artery occluded (MCAo) rat brain. In vitro, 4-weeks after removing growth factors and 4-OHT from the culture medium, c-mycERTAM transgene transcription is reduced by ~75%. Furthermore, immunocytochemistry and western blotting demonstrated a concurrent decrease in the c-MycERTAM protein. To examine the transcription of the transgene in vivo, CTX0E03 cells (450,000) were implanted 4-weeks post MCAo lesion and analysed for human cell survival and c-mycERTAM transcription by qPCR and qRT-PCR, respectively. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 2% |
| Ireland | 1 | 2% |
| France | 1 | 2% |
| Belgium | 1 | 2% |
| Unknown | 52 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 25% |
| Researcher | 11 | 20% |
| Student > Bachelor | 10 | 18% |
| Other | 6 | 11% |
| Student > Master | 5 | 9% |
| Other | 4 | 7% |
| Unknown | 6 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 30% |
| Medicine and Dentistry | 12 | 21% |
| Biochemistry, Genetics and Molecular Biology | 4 | 7% |
| Engineering | 3 | 5% |
| Chemical Engineering | 2 | 4% |
| Other | 6 | 11% |
| Unknown | 12 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 June 2012.
All research outputs
#5,957,595
of 22,669,724 outputs
Outputs from BMC Neuroscience
#280
of 1,240 outputs
Outputs of similar age
#31,899
of 110,458 outputs
Outputs of similar age from BMC Neuroscience
#11
of 28 outputs
Altmetric has tracked 22,669,724 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 1,240 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 110,458 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.