Title |
Rapid genome-scale mapping of chromatin accessibility in tissue
|
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Published in |
Epigenetics & Chromatin, June 2012
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DOI | 10.1186/1756-8935-5-10 |
Pubmed ID | |
Authors |
Lars Grøntved, Russell Bandle, Sam John, Songjoon Baek, Hye-Jung Chung, Ying Liu, Greti Aguilera, Carl Oberholtzer, Gordon L Hager, David Levens |
Abstract |
The challenge in extracting genome-wide chromatin features from limiting clinical samples poses a significant hurdle in identification of regulatory marks that impact the physiological or pathological state. Current methods that identify nuclease accessible chromatin are reliant on large amounts of purified nuclei as starting material. This complicates analysis of trace clinical tissue samples that are often stored frozen. We have developed an alternative nuclease based procedure to bypass nuclear preparation to interrogate nuclease accessible regions in frozen tissue samples. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
United States | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 4 | 5% |
Portugal | 1 | 1% |
Denmark | 1 | 1% |
Germany | 1 | 1% |
Unknown | 81 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 40 | 45% |
Student > Ph. D. Student | 21 | 24% |
Professor > Associate Professor | 5 | 6% |
Student > Bachelor | 3 | 3% |
Other | 3 | 3% |
Other | 10 | 11% |
Unknown | 6 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 51 | 58% |
Biochemistry, Genetics and Molecular Biology | 26 | 30% |
Medicine and Dentistry | 4 | 5% |
Computer Science | 2 | 2% |
Unknown | 5 | 6% |