Title |
Phenotypic characterization and 16S rDNA identification of culturable non-obligate halophilic bacterial communities from a hypersaline lake, La Sal del Rey, in extreme South Texas (USA)
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Published in |
Aquatic Biosystems, February 2012
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DOI | 10.1186/2046-9063-8-5 |
Pubmed ID | |
Authors |
Kristen Phillips, Frederic Zaidan, Omar R Elizondo, Kristine L Lowe |
Abstract |
La Sal del Rey ("the King's Salt") is one of several naturally-occurring salt lakes in Hidalgo County, Texas and is part of the Lower Rio Grande Valley National Wildlife Refuge. The research objective was to isolate and characterize halophilic microorganisms from La Sal del Rey. Water samples were collected from the lake and a small creek that feeds into the lake. Soil samples were collected from land adjacent to the water sample locations. Sample salinity was determined using a refractometer. Samples were diluted and cultured on a synthetic saline medium to grow halophilic bacteria. The density of halophiles was estimated by viable plate counts. A collection of isolates was selected, gram-stained, tested for catalase, and characterized using API 20E® test strips. Isolates were putatively identified by sequencing the 16S rDNA. Carbon source utilization by the microbial community from each sample site was examined using EcoPlate™ assays and the carbon utilization total activity of the community was determined. |
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Student > Doctoral Student | 6 | 13% |
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Student > Bachelor | 4 | 9% |
Other | 9 | 19% |
Unknown | 3 | 6% |
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Environmental Science | 4 | 9% |
Immunology and Microbiology | 4 | 9% |
Earth and Planetary Sciences | 3 | 6% |
Other | 3 | 6% |
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