Title |
Proteomic analysis of short-term preload-induced eccentric cardiac hypertrophy
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Published in |
Journal of Translational Medicine, May 2016
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DOI | 10.1186/s12967-016-0898-5 |
Pubmed ID | |
Authors |
Belal A. Mohamed, Abdul R. Asif, Moritz Schnelle, Mohamed Qasim, Sara Khadjeh, Dawid Lbik, Peter Schott, Gerd Hasenfuss, Karl Toischer |
Abstract |
Hemodynamic load leads to cardiac hypertrophy and heart failure. While afterload (pressure overload) induces concentric hypertrophy, elevation of preload (volume overload) yields eccentric hypertrophy and is associated with a better outcome. Here we analysed the proteomic pattern of mice subjected to short-term preload. Female FVB/N mice were subjected to aortocaval shunt-induced volume overload that leads to an eccentric hypertrophy (left ventricular weight/tibia length +31 %) with sustained systolic heart function at 1 week after operation. Two-dimensional gel electrophoresis (2-DE) followed by mass spectrometric analysis showed alteration in the expression of 25 protein spots representing 21 different proteins. 64 % of these protein spots were up-regulated and 36 % of the protein spots were consistently down-regulated. Interestingly, α-1-antitrypsin was down-regulated, indicating higher elastin degradation and possibly contributing to the early dilatation. In addition to contractile and mitochondrial proteins, polymerase I and transcript release factor protein (PTRF) was also up-regulated, possibly contributing to the preload-induced signal transduction. Our findings reveal the proteomic changes of early-stage eccentric myocardial remodeling after volume overload. Induced expression of some of the respiratory chain enzymes suggests a metabolic shift towards an oxidative phosphorylation that might contribute to the favorable remodeling seen in early VO. Down-regulation of α-1-antitrypsin might contribute to extracellular matrix remodeling and left ventricular dilatation. We also identified PTRF as a potential signaling regulator of volume overload-induced cardiac hypertrophy. |
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Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 21% |
Student > Master | 4 | 17% |
Professor > Associate Professor | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 1 | 4% |
Other | 3 | 13% |
Unknown | 7 | 29% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 25% |
Medicine and Dentistry | 3 | 13% |
Unspecified | 1 | 4% |
Arts and Humanities | 1 | 4% |
Sports and Recreations | 1 | 4% |
Other | 1 | 4% |
Unknown | 11 | 46% |