Title |
Identification of soybean seed developmental stage-specific and tissue-specific miRNA targets by degradome sequencing
|
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Published in |
BMC Genomics, July 2012
|
DOI | 10.1186/1471-2164-13-310 |
Pubmed ID | |
Authors |
Md Shamimuzzaman, Lila Vodkin |
Abstract |
MicroRNAs (miRNAs) regulate the expression of target genes by mediating gene silencing in both plants and animals. The miRNA targets have been extensively investigated in Arabidopsis and rice using computational prediction, experimental validation by overexpression in transgenic plants, and by degradome or PARE (parallel analysis of RNA ends) sequencing. However, miRNA targets mostly remain unknown in soybean (Glycine max). More specifically miRNA mediated gene regulation at different seed developmental stages in soybean is largely unexplored. In order to dissect miRNA guided gene regulation in soybean developing seeds, we performed a transcriptome-wide experimental method using degradome sequencing to directly detect cleaved miRNA targets. |
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