Title |
Evaluation of antigen detection and polymerase chain reaction for diagnosis of amoebic liver abscess in patients on anti-amoebic treatment
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Published in |
BMC Research Notes, August 2012
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DOI | 10.1186/1756-0500-5-416 |
Pubmed ID | |
Authors |
Virendra Jaiswal, Ujjala Ghoshal, Sanjay S Baijal, Balraj Mittal, Tapan N Dhole, Uday C Ghoshal |
Abstract |
Diagnosis of amoebic liver abscess (ALA) in patients on anti-amoebic drugs is difficult. There is scanty data on this issue using Entamoeba histolytica (E. histolytica) lectin antigen and polymerase chain reaction (PCR). We studied utility of lectin antigen, PCR, and IgG antibody in diagnosis of liver abscess in patients on anti-amoebic treatment. Liver aspirate of 200 patients, of which 170 had anti-amoebic drug prior to drainage, was tested for E. histolytica lectin antigen by (ELISA), PCR, bacterial culture, and serum IgG antibody by (ELISA). Classification of abscesses was based on result of anti-amoebic IgG antibody and bacterial culture, E. histolytica PCR and bacterial culture, and E. histolytica lectin antigen and bacterial culture. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 3 | 17% |
Professor | 2 | 11% |
Other | 2 | 11% |
Student > Postgraduate | 2 | 11% |
Student > Master | 2 | 11% |
Other | 3 | 17% |
Unknown | 4 | 22% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 4 | 22% |
Biochemistry, Genetics and Molecular Biology | 3 | 17% |
Medicine and Dentistry | 3 | 17% |
Agricultural and Biological Sciences | 2 | 11% |
Social Sciences | 1 | 6% |
Other | 0 | 0% |
Unknown | 5 | 28% |