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Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system

Overview of attention for article published in BMC Genomics, August 2012
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2 X users

Citations

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39 Dimensions

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102 Mendeley
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Title
Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system
Published in
BMC Genomics, August 2012
DOI 10.1186/1471-2164-13-385
Pubmed ID
Authors

César Aguilar, Adelfo Escalante, Noemí Flores, Ramón de Anda, Fernando Riveros-McKay, Guillermo Gosset, Enrique Morett, Francisco Bolívar

Abstract

Escherichia coli strains lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS), which is the major bacterial component involved in glucose transport and its phosphorylation, accumulate high amounts of phosphoenolpyruvate that can be diverted to the synthesis of commercially relevant products. However, these strains grow slowly in glucose as sole carbon source due to its inefficient transport and metabolism. Strain PB12, with 400% increased growth rate, was isolated after a 120 hours adaptive laboratory evolution process for the selection of faster growing derivatives in glucose. Analysis of the genetic changes that occurred in the PB12 strain that lacks PTS will allow a better understanding of the basis of its growth adaptation and, therefore, in the design of improved metabolic engineering strategies for enhancing carbon diversion into the aromatic pathways.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 2 2%
Mexico 2 2%
Switzerland 1 <1%
Australia 1 <1%
Portugal 1 <1%
Sweden 1 <1%
Estonia 1 <1%
United States 1 <1%
Unknown 92 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 25%
Researcher 23 23%
Student > Master 18 18%
Professor 6 6%
Student > Doctoral Student 5 5%
Other 15 15%
Unknown 10 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 58 57%
Biochemistry, Genetics and Molecular Biology 24 24%
Engineering 4 4%
Chemical Engineering 1 <1%
Mathematics 1 <1%
Other 4 4%
Unknown 10 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2012.
All research outputs
#15,523,357
of 24,598,501 outputs
Outputs from BMC Genomics
#5,986
of 11,014 outputs
Outputs of similar age
#104,799
of 173,284 outputs
Outputs of similar age from BMC Genomics
#66
of 111 outputs
Altmetric has tracked 24,598,501 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,014 research outputs from this source. They receive a mean Attention Score of 4.8. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 173,284 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 111 others from the same source and published within six weeks on either side of this one. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.