@gibbological @mikemc423 @bejcal @AmyDWillis This is one reason that spike-in quantification is so promising! (e.g. here in 16S, but also works in shotgun) https://t.co/kY4Kdu19XJ
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@DaniVadim 2/2 Based on this paper: https://t.co/bp3otjcd0r This essentially allows you to get the 16S copy number in your samples.
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@davidacoil @ZymoResearch https://t.co/uo45s4G6uF uses cellular spike-ins for this. Unclear to me how useful DNA spike-ins are b/c of extraction bias.
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@DNAkendra @MikeyJ You might also check out https://t.co/ZSGedGr3aZ as something similar. I'd be interested in something like this if it's trustworthy