Title |
PPARGC1α gene DNA methylation variations in human placenta mediate the link between maternal hyperglycemia and leptin levels in newborns
|
---|---|
Published in |
Clinical Epigenetics, June 2016
|
DOI | 10.1186/s13148-016-0239-9 |
Pubmed ID | |
Authors |
Sandra Côté, Valérie Gagné-Ouellet, Simon-Pierre Guay, Catherine Allard, Andrée-Anne Houde, Patrice Perron, Jean-Patrice Baillargeon, Daniel Gaudet, Renée Guérin, Diane Brisson, Marie-France Hivert, Luigi Bouchard |
Abstract |
Children exposed to gestational diabetes mellitus (GDM) are at a higher risk of developing obesity and type 2 diabetes. This susceptibility might involve brown adipose tissue (BAT), which is suspected to protect against obesity. The objective of this study is to assess whether fetal exposure to maternal hyperglycemia is associated with DNA methylation variations in genes involved in BAT genesis and activation. DNA methylation levels at the PRDM16, BMP7, CTBP2, and PPARGC1α gene loci were measured in placenta samples using bisulfite pyrosequencing in E-21 (n = 133; 33 cases of GDM) and the HumanMethylation450 array in Gen3G (n = 172, all from non-diabetic women) birth cohorts. Glucose tolerance was assessed in all women using an oral glucose tolerance test at the second trimester of pregnancy. Participating women were extensively phenotyped throughout pregnancy, and placenta and cord blood samples were collected at birth. We report that maternal glycemia at the second and third trimester of pregnancy are correlated with variations in DNA methylation levels at PRDM16, BMP7, and PPARGC1α and with cord blood leptin levels. Variations in PRDM16 and PPARGC1α DNA methylation levels were also correlated with cord blood leptin levels. Mediation analyses support that DNA methylation variations at the PPARGC1α gene locus explain 0.8 % of the cord blood leptin levels variance independently of maternal fasting glucose levels (p = 0.05). These results suggest that maternal glucose in pregnancy could produce variations in DNA methylation in BAT-related genes and that some of these DNA methylation marks seem to mediate the impact of maternal glycemia on cord blood leptin levels, an adipokine regulating body weight. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 2 | 40% |
United Kingdom | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 60% |
Scientists | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 96 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 21% |
Student > Bachelor | 16 | 17% |
Student > Master | 15 | 16% |
Researcher | 7 | 7% |
Student > Doctoral Student | 6 | 6% |
Other | 16 | 17% |
Unknown | 16 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 27 | 28% |
Biochemistry, Genetics and Molecular Biology | 24 | 25% |
Nursing and Health Professions | 9 | 9% |
Agricultural and Biological Sciences | 5 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
Other | 8 | 8% |
Unknown | 20 | 21% |