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Optimized methods for extracting circulating small RNAs from long-term stored equine samples

Overview of attention for article published in Acta Veterinaria Scandinavica, June 2016
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  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

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3 X users

Citations

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Title
Optimized methods for extracting circulating small RNAs from long-term stored equine samples
Published in
Acta Veterinaria Scandinavica, June 2016
DOI 10.1186/s13028-016-0224-5
Pubmed ID
Authors

Lucia Unger, Nathalie Fouché, Tosso Leeb, Vincent Gerber, Alicja Pacholewska

Abstract

Circulating miRNAs in body fluids, particularly serum, are promising candidates for future routine biomarker profiling in various pathologic conditions in human and veterinary medicine. However, reliable standardized methods for miRNA extraction from equine serum and fresh or archived whole blood are sorely lacking. We systematically compared various miRNA extraction methods from serum and whole blood after short and long-term storage without addition of RNA stabilizing additives prior to freezing. Time of storage at room temperature prior to freezing did not affect miRNA quality in serum. Furthermore, we showed that miRNA of NGS-sufficient quality can be recovered from blood samples after >10 years of storage at -80 °C. This allows retrospective analyses of miRNAs from archived samples.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 27%
Student > Ph. D. Student 4 15%
Student > Master 4 15%
Student > Bachelor 3 12%
Unspecified 2 8%
Other 3 12%
Unknown 3 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 23%
Veterinary Science and Veterinary Medicine 4 15%
Agricultural and Biological Sciences 3 12%
Unspecified 2 8%
Medicine and Dentistry 2 8%
Other 6 23%
Unknown 3 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2016.
All research outputs
#17,285,036
of 25,371,288 outputs
Outputs from Acta Veterinaria Scandinavica
#439
of 837 outputs
Outputs of similar age
#237,452
of 367,282 outputs
Outputs of similar age from Acta Veterinaria Scandinavica
#7
of 16 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 837 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 367,282 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.