Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

Overview of attention for article published in BMC Bioinformatics, September 2020

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Why is there a minus in the 2ˆ(–delta delta CT) method (qPCR)

Posted by Paul, at bioinformatics.stackexchange.com, 09 Nov 2020

Question: Why is there a minus in the 2ˆ(–delta delta CT) formula? My line of thoughs: Consider Ct values of some pPCR…

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