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Protein stability regulators screening assay (Pro‐SRSA): protein degradation meets the CRISPR–Cas9 library

Overview of attention for article published in Cancer Communications, June 2016
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Title
Protein stability regulators screening assay (Pro‐SRSA): protein degradation meets the CRISPR–Cas9 library
Published in
Cancer Communications, June 2016
DOI 10.1186/s40880-016-0125-z
Pubmed ID
Authors

Yuanzhong Wu, Tiebang Kang

Abstract

The regulation of protein stability is a fundamental issue for biophysical processes, but there has not previously been a convenient and unbiased method of identifying regulators of protein stability. However, as reported in the article entitled "A genome-scale CRISPR-Cas9 screening method for protein stability reveals novel regulators of Cdc25A," recently published in Cell Discovery, our team developed a protein stability regulators screening assay (Pro-SRSA) by combining the whole-genome clustered regularly interspaced short palindromic repeats Cas9 (CRISPR-Cas9) library with a dual-fluorescence-based protein stability reporter and high-throughput sequencing to screen for regulators of protein stability. Based on our findings, we are confident that this efficient and unbiased screening method at the genome scale will be used by researchers worldwide to identify regulators of protein stability.

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Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 32%
Researcher 3 16%
Other 2 11%
Student > Bachelor 1 5%
Student > Master 1 5%
Other 1 5%
Unknown 5 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 32%
Agricultural and Biological Sciences 4 21%
Immunology and Microbiology 2 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Medicine and Dentistry 1 5%
Other 2 11%
Unknown 3 16%