A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti

Nil Rahola, Leila Hadj Henni, Judicael Obame, Diego Ayala, Boris Kevin Makanga, Véronique Lehrter, Arezki Izri, Christophe Paupy, Jérôme Depaquit

The genus Spelaeomyia includes four African species considered as being cavernicolous: Spelaeomyia darlingi, Spelaeomyia mirabilis, Spelaeomyia emilii and Spelaeomyia moucheti. Despite a potential role in Leishmania major leishmaniasis transmission in Mali, no molecular studies and only few morphological studies have addressed relationships between species of Spelaeomyia. Specimens of Sa. moucheti were collected in two different sites in Gabon. Spelaeomyia emilii and Sa. darlingi specimens came from Gabon and Mali. Specimens of Sa. mirabilis were collected in the Democratic Republic of Congo and Gabon. All specimens were caught using CDC miniature light traps, then dissected, both heads and genitalia were kept for morphological analysis and the rest of the bodies were kept for molecular processing and analyses. Some unidentified males are associated to Sa. moucheti females using molecular tools and are described for the first time. A new morphological feature is observed on the spermathecae of the female and new drawings are provided. For the first time a phylogenetic analysis is carried out on rDNA and mtDNA markers and it shows that Sa. moucheti is the sister species of Sa. mirabilis. Spelaeomyia moucheti is the sister species of Sa. mirabilis. This result is in agreement with the sharing of morphological characters between these closely related species. Moreover, these two species are not as cavernicolous as literature previously indicated. They were caught in open rainforest in Gabon.