Title |
GiniClust: detecting rare cell types from single-cell gene expression data with Gini index
|
---|---|
Published in |
Genome Biology, July 2016
|
DOI | 10.1186/s13059-016-1010-4 |
Pubmed ID | |
Authors |
Lan Jiang, Huidong Chen, Luca Pinello, Guo-Cheng Yuan |
Abstract |
High-throughput single-cell technologies have great potential to discover new cell types; however, it remains challenging to detect rare cell types that are distinct from a large population. We present a novel computational method, called GiniClust, to overcome this challenge. Validation against a benchmark dataset indicates that GiniClust achieves high sensitivity and specificity. Application of GiniClust to public single-cell RNA-seq datasets uncovers previously unrecognized rare cell types, including Zscan4-expressing cells within mouse embryonic stem cells and hemoglobin-expressing cells in the mouse cortex and hippocampus. GiniClust also correctly detects a small number of normal cells that are mixed in a cancer cell population. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 4 | 20% |
United States | 3 | 15% |
Belgium | 1 | 5% |
Brazil | 1 | 5% |
South Africa | 1 | 5% |
Australia | 1 | 5% |
France | 1 | 5% |
Unknown | 8 | 40% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 12 | 60% |
Members of the public | 7 | 35% |
Science communicators (journalists, bloggers, editors) | 1 | 5% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 1% |
Japan | 2 | <1% |
Brazil | 1 | <1% |
Germany | 1 | <1% |
United Kingdom | 1 | <1% |
Unknown | 286 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 70 | 24% |
Researcher | 50 | 17% |
Student > Bachelor | 26 | 9% |
Student > Postgraduate | 20 | 7% |
Student > Master | 20 | 7% |
Other | 48 | 16% |
Unknown | 61 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 78 | 26% |
Agricultural and Biological Sciences | 75 | 25% |
Computer Science | 35 | 12% |
Engineering | 8 | 3% |
Neuroscience | 7 | 2% |
Other | 27 | 9% |
Unknown | 65 | 22% |