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A review of COFRADIC techniques targeting protein N-terminal acetylation

Overview of attention for article published in BMC Proceedings, August 2009
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Title
A review of COFRADIC techniques targeting protein N-terminal acetylation
Published in
BMC Proceedings, August 2009
DOI 10.1186/1753-6561-3-s6-s6
Pubmed ID
Authors

Petra Van Damme, Jozef Van Damme, Hans Demol, An Staes, Joël Vandekerckhove, Kris Gevaert

Abstract

Acetylation of nascent protein Nalpha-termini is a common modification among archae and eukaryotes and can influence the structure and function of target proteins. This modification has been studied on an individual protein or (synthetic) peptide level or on a proteome scale using two-dimensional polyacrylamide gel electrophoresis. We recently developed mass spectrometry driven proteome analytical approaches specifically targeting the amino (N) terminus of proteins based on the concept of diagonal reverse-phase chromatography. We here review how this so-called combined fractional diagonal chromatography (COFRADIC) technique can be used in combination with differential mass-tagging strategies as to both qualitatively and quantitatively assess protein Nalpha-acetylation in whole proteomes.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 3%
Norway 2 2%
Netherlands 1 1%
United States 1 1%
Unknown 92 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 26 26%
Researcher 20 20%
Student > Master 16 16%
Student > Bachelor 8 8%
Student > Doctoral Student 6 6%
Other 19 19%
Unknown 4 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 43 43%
Biochemistry, Genetics and Molecular Biology 19 19%
Chemistry 10 10%
Medicine and Dentistry 7 7%
Computer Science 4 4%
Other 8 8%
Unknown 8 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2012.
All research outputs
#15,249,959
of 22,675,759 outputs
Outputs from BMC Proceedings
#209
of 374 outputs
Outputs of similar age
#93,907
of 110,765 outputs
Outputs of similar age from BMC Proceedings
#5
of 7 outputs
Altmetric has tracked 22,675,759 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 374 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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