Title |
A review of COFRADIC techniques targeting protein N-terminal acetylation
|
---|---|
Published in |
BMC Proceedings, August 2009
|
DOI | 10.1186/1753-6561-3-s6-s6 |
Pubmed ID | |
Authors |
Petra Van Damme, Jozef Van Damme, Hans Demol, An Staes, Joël Vandekerckhove, Kris Gevaert |
Abstract |
Acetylation of nascent protein Nalpha-termini is a common modification among archae and eukaryotes and can influence the structure and function of target proteins. This modification has been studied on an individual protein or (synthetic) peptide level or on a proteome scale using two-dimensional polyacrylamide gel electrophoresis. We recently developed mass spectrometry driven proteome analytical approaches specifically targeting the amino (N) terminus of proteins based on the concept of diagonal reverse-phase chromatography. We here review how this so-called combined fractional diagonal chromatography (COFRADIC) technique can be used in combination with differential mass-tagging strategies as to both qualitatively and quantitatively assess protein Nalpha-acetylation in whole proteomes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 3% |
Norway | 2 | 2% |
Netherlands | 1 | 1% |
United States | 1 | 1% |
Unknown | 92 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 26 | 26% |
Researcher | 20 | 20% |
Student > Master | 16 | 16% |
Student > Bachelor | 8 | 8% |
Student > Doctoral Student | 6 | 6% |
Other | 19 | 19% |
Unknown | 4 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 43 | 43% |
Biochemistry, Genetics and Molecular Biology | 19 | 19% |
Chemistry | 10 | 10% |
Medicine and Dentistry | 7 | 7% |
Computer Science | 4 | 4% |
Other | 8 | 8% |
Unknown | 8 | 8% |