Title |
Characterization of functional mannose receptor in a continuous hybridoma cell line
|
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Published in |
BMC Immunology, September 2012
|
DOI | 10.1186/1471-2172-13-51 |
Pubmed ID | |
Authors |
David J Vigerust, Sherell Vick, Virginia L Shepherd |
Abstract |
The mannose receptor is the best described member of the type I transmembrane C-type lectins; however much remains unanswered about the biology of the receptor. One difficulty has been the inability to consistently express high levels of a functional full length mannose receptor cDNA in mammalian cells. Another difficulty has been the lack of a human macrophage cell line expressing a fully functional receptor. Commonly used human macrophage cell lines such as U937, THP-1, Mono-Mac and HL60 do not express the mannose receptor. We have developed a macrophage hybridoma cell line (43MR cells) created by fusion of U937 cells with primary human monocyte-derived macrophages, resulting in a non-adherent cell line expressing several properties of primary macrophages. The purpose of this study was to identify and select mannose receptor-expressing cells using fluorescence-activated cell sorting and to characterize the expression and function of the receptor. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Argentina | 1 | 2% |
Unknown | 65 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 16 | 24% |
Student > Bachelor | 10 | 15% |
Researcher | 10 | 15% |
Student > Master | 10 | 15% |
Student > Doctoral Student | 3 | 5% |
Other | 6 | 9% |
Unknown | 11 | 17% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 16 | 24% |
Biochemistry, Genetics and Molecular Biology | 12 | 18% |
Medicine and Dentistry | 7 | 11% |
Immunology and Microbiology | 7 | 11% |
Chemistry | 4 | 6% |
Other | 7 | 11% |
Unknown | 13 | 20% |