Title |
Ubiquitin initiates sorting of Golgi and plasma membrane proteins into the vacuolar degradation pathway
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Published in |
BMC Plant Biology, September 2012
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DOI | 10.1186/1471-2229-12-164 |
Pubmed ID | |
Authors |
David Scheuring, Fabian Künzl, Corrado Viotti, Melody SanWan Yan, Liwen Jiang, Swen Schellmann, David G Robinson, Peter Pimpl |
Abstract |
In yeast and mammals, many plasma membrane (PM) proteins destined for degradation are tagged with ubiquitin. These ubiquitinated proteins are internalized into clathrin-coated vesicles and are transported to early endosomal compartments. There, ubiquitinated proteins are sorted by the endosomal sorting complex required for transport (ESCRT) machinery into the intraluminal vesicles of multivesicular endosomes. Degradation of these proteins occurs after endosomes fuse with lysosomes/lytic vacuoles to release their content into the lumen. In plants, some PM proteins, which cycle between the PM and endosomal compartments, have been found to be ubiquitinated, but it is unclear whether ubiquitin is sufficient to mediate internalization and thus acts as a primary sorting signal for the endocytic pathway. To test whether plants use ubiquitin as a signal for the degradation of membrane proteins, we have translationally fused ubiquitin to different fluorescent reporters for the plasma membrane and analyzed their transport. |
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Demographic breakdown
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Mendeley readers
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Mexico | 1 | <1% |
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Researcher | 19 | 19% |
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Professor > Associate Professor | 5 | 5% |
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Immunology and Microbiology | 1 | <1% |
Other | 1 | <1% |
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