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X Demographics
Mendeley readers
Attention Score in Context
| Title |
In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins
|
|---|---|
| Published in |
BMC Biotechnology, September 2012
|
| DOI | 10.1186/1472-6750-12-63 |
| Pubmed ID | |
| Authors |
Jason S Buhrman, Jamie E Rayahin, Melanie Köllmer, Richard A Gemeinhart |
| Abstract |
Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular proteins in lysates. Although GST affinity materials are commercially available as glutathione immobilized on beaded agarose resins, few simple options for in-house production of those systems exist. Herein, we describe a novel method for the purification of GST-tagged recombinant proteins. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | 3% |
| Unknown | 28 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 28% |
| Student > Bachelor | 5 | 17% |
| Student > Ph. D. Student | 4 | 14% |
| Professor | 3 | 10% |
| Student > Doctoral Student | 3 | 10% |
| Other | 2 | 7% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 9 | 31% |
| Agricultural and Biological Sciences | 5 | 17% |
| Biochemistry, Genetics and Molecular Biology | 3 | 10% |
| Materials Science | 2 | 7% |
| Neuroscience | 2 | 7% |
| Other | 3 | 10% |
| Unknown | 5 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 September 2012.
All research outputs
#15,251,976
of 22,679,690 outputs
Outputs from BMC Biotechnology
#668
of 935 outputs
Outputs of similar age
#107,206
of 170,567 outputs
Outputs of similar age from BMC Biotechnology
#14
of 18 outputs
Altmetric has tracked 22,679,690 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 935 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 170,567 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.