Title |
In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications
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Published in |
Journal of Nanobiotechnology, November 2015
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DOI | 10.1186/s12951-015-0151-z |
Pubmed ID | |
Authors |
Kyoung Suk Kang, Hyun Uk Lee, Moon Il Kim, So Young Park, Sung-Jin Chang, Ji-Ho Park, Yun Suk Huh, Jouhahn Lee, Mino Yang, Young-Chul Lee, Hyun Gyu Park |
Abstract |
We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role in bio-imaging and FeAC NPs act as a substrate for CD conjugation and help to uptake of NPs into cancer cells due to positively charged surface of FeAC NPs in physiological media. As increase of CD-FeAC NPs loading in HeLa cell in vitro, it showed slight cytotoxicity at 1000 μg/mL but no cytotoxicity for normal cells up to concentration of 1000 μg/mL confirmed by two 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, with further observations by 4',6-diamidino-2-phenylindole (DAPI) stained confocal microscopy images, possessing that CD-FeAC NPs can be used as potential drug delivery platforms in cancer cells with simultaneous imaging. Graphical abstract CD conjugation with organo-building blocks of delaminated FeAC NPs. |
X Demographics
Geographical breakdown
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Portugal | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 41 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 10 | 24% |
Researcher | 7 | 17% |
Student > Ph. D. Student | 6 | 15% |
Unspecified | 2 | 5% |
Student > Doctoral Student | 2 | 5% |
Other | 4 | 10% |
Unknown | 10 | 24% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 12% |
Chemistry | 4 | 10% |
Engineering | 4 | 10% |
Materials Science | 4 | 10% |
Chemical Engineering | 3 | 7% |
Other | 10 | 24% |
Unknown | 11 | 27% |