Title |
QUAliFiER: An automated pipeline for quality assessment of gated flow cytometry data
|
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Published in |
BMC Bioinformatics, September 2012
|
DOI | 10.1186/1471-2105-13-252 |
Pubmed ID | |
Authors |
Greg Finak, Wenxin Jiang, Jorge Pardo, Adam Asare, Raphael Gottardo |
Abstract |
Effective quality assessment is an important part of any high-throughput flow cytometry data analysis pipeline, especially when considering the complex designs of the typical flow experiments applied in clinical trials. Technical issues like instrument variation, problematic antibody staining, or reagent lot changes can lead to biases in the extracted cell subpopulation statistics. These biases can manifest themselves in non-obvious ways that can be difficult to detect without leveraging information about the study design or other experimental metadata. Consequently, a systematic and integrated approach to quality assessment of flow cytometry data is necessary to effectively identify technical errors that impact multiple samples over time. Gated cell populations and their statistics must be monitored within the context of the experimental run, assay, and the overall study. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 50% |
Canada | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 50% |
Members of the public | 1 | 25% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 4% |
United Kingdom | 1 | 2% |
Estonia | 1 | 2% |
Germany | 1 | 2% |
Unknown | 44 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 18 | 37% |
Student > Ph. D. Student | 7 | 14% |
Student > Bachelor | 5 | 10% |
Professor > Associate Professor | 4 | 8% |
Other | 2 | 4% |
Other | 6 | 12% |
Unknown | 7 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 37% |
Computer Science | 6 | 12% |
Immunology and Microbiology | 4 | 8% |
Medicine and Dentistry | 3 | 6% |
Engineering | 3 | 6% |
Other | 7 | 14% |
Unknown | 8 | 16% |