Review by Antonio Gonzalez Pena
I believe the changes made by the authors have improved the manuscript greatly and is ready for publication. Level of interest: An article of importance in its…
I believe the changes made by the authors have improved the manuscript greatly and is ready for publication. Level of interest: An article of importance in its…
Major Compulsory Revisions: The authors acknowledge that their tests on revealing abundance information could be improved by using well-designed tests but I believe that this could improve the full…
In this paper, the authors present an interesting and timely experiment. They demonstrate the exciting potential for direct (without PCR) sequencing on the Illumina platform to reveal species…
This manuscript corresponds to the first "PCR-free" DNA metabarcoding experiment for analyzing bulk animal samples. I fully agree with the authors that PCR has many drawbacks during classical DNA…