Title |
SPINGO: a rapid species-classifier for microbial amplicon sequences
|
---|---|
Published in |
BMC Bioinformatics, October 2015
|
DOI | 10.1186/s12859-015-0747-1 |
Pubmed ID | |
Authors |
Guy Allard, Feargal J. Ryan, Ian B. Jeffery, Marcus J. Claesson |
Abstract |
Taxonomic classification is a corner stone for the characterisation and comparison of microbial communities. Currently, most existing methods are either slow, restricted to specific communities, highly sensitive to taxonomic inconsistencies, or limited to genus level classification. As crucial microbiota information is hinging on high-level resolution it is imperative to increase taxonomic resolution to species level wherever possible. In response to this need we developed SPINGO, a flexible and stand-alone software dedicated to high-resolution assignment of sequences to species level using partial 16S rRNA gene sequences from any environment. SPINGO compares favourably to other methods in terms of classification accuracy, and is as fast or faster than those that have higher error rates. As a demonstration of its flexibility for other types of target genes we successfully applied SPINGO also on cpn60 amplicon sequences. SPINGO is an accurate, flexible and fast method for low-level taxonomic assignment. This combination is becoming increasingly important for rapid and accurate processing of amplicon data generated by newer next generation sequencing technologies. |
Twitter Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 2 | 13% |
Germany | 2 | 13% |
United Kingdom | 1 | 6% |
United States | 1 | 6% |
Finland | 1 | 6% |
Italy | 1 | 6% |
France | 1 | 6% |
Unknown | 7 | 44% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 10 | 63% |
Members of the public | 6 | 38% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 3 | 2% |
France | 2 | 1% |
United States | 2 | 1% |
Ireland | 1 | <1% |
Sweden | 1 | <1% |
Canada | 1 | <1% |
United Kingdom | 1 | <1% |
Spain | 1 | <1% |
Mexico | 1 | <1% |
Other | 0 | 0% |
Unknown | 126 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 37 | 27% |
Researcher | 32 | 23% |
Student > Master | 16 | 12% |
Student > Bachelor | 9 | 6% |
Student > Doctoral Student | 6 | 4% |
Other | 20 | 14% |
Unknown | 19 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 38 | 27% |
Biochemistry, Genetics and Molecular Biology | 21 | 15% |
Immunology and Microbiology | 17 | 12% |
Environmental Science | 10 | 7% |
Computer Science | 8 | 6% |
Other | 15 | 11% |
Unknown | 30 | 22% |